| Primer | Species | LP | RP | Tml | Tmr | Length | Score | Target | Senstive |
|---|---|---|---|---|---|---|---|---|---|
| Primer 1 | Nitrobacter winogradskyi | TCTATCAGCCGACGCACAC | CACCGAAAACGGCTTGGTG | 59.86 | 60.01 | 205 | 127 |
100.00%
|
100.00%
|
| Primer 2 | Nitrobacter winogradskyi | CGTTTGAAGACGGCAGAGC | AGGCTTGCTTGGCGAGAT | 59.51 | 59.33 | 162 | 116 |
100.00%
|
100.00%
|
| Primer 3 | Nitrobacter winogradskyi | GCGAGCGGTTTTCAGAAGG | AGGCTTGCTTGGCGAGAT | 59.50 | 59.33 | 226 | 116 |
100.00%
|
100.00%
|
| Primer 4 | Nitrobacter winogradskyi | TGACGAAAGACGAGGGCAG | AGCACGCACCCTTCATGT | 59.71 | 59.57 | 291 | 115 |
100.00%
|
100.00%
|
| Primer 5 | Nitrobacter winogradskyi | CGTGCAATTCGTCGGCAT | TCAGCACCGCTTCGATGT | 59.22 | 59.34 | 227 | 115 |
100.00%
|
100.00%
|
| Primer 6 | Nitrobacter winogradskyi | CGCGAGCGGTTTTCAGAAG | AGGCTTGCTTGGCGAGAT | 59.87 | 59.33 | 227 | 115 |
100.00%
|
100.00%
|
| Primer 7 | Nitrobacter winogradskyi | ATGTCCGGTCGACTGCTTC | ATGTTTTCGCGTCGGCAG | 59.78 | 59.14 | 218 | 115 |
100.00%
|
100.00%
|
| Primer 8 | Nitrobacter winogradskyi | TTGGATGCCTGCGGATCAG | AGGCTTGCTCTTGGTGACC | 60.15 | 59.93 | 169 | 114 |
100.00%
|
100.00%
|
| Primer 9 | Nitrobacter winogradskyi | ACAGCTTCTCGCCTTCGTC | TGGCGTCGAAGCGAAACT | 60.08 | 59.97 | 158 | 113 |
100.00%
|
100.00%
|
| Primer 10 | Nitrobacter winogradskyi | TGTCGCTGTCACAAGGCA | TGTCGGGATCGCCATTGT | 59.50 | 59.01 | 231 | 113 |
100.00%
|
100.00%
|